Date of Award

2016

Document Type

Thesis

Keywords

American chestnut

Abstract

The American chestnut tree was once a common and important species. It has been reduced to an understory shrub by a fungal pathogen. This decimation of a once majestic tree prompted considerable restoration efforts. These have culminated in transferring genetic resistance to the chestnut by transformation with Agrobacterium. The past events (genetic lines from a single transformed cell) have not been shown to vary significantly in root colonization by ectomycorrhizal fungi. This experiment used three recent events to assess colonization. There was no significant variation among two of the events and the American chestnut. The significantly different event was explained by confounding experimental factors.

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