Date of Award
Spring 5-13-2017
Semester of Degree
May
Document Type
Open Access Thesis
Degree Name
M.S. in Environmental and Forest Biology
Department
Environmental and Forest Biology
Major Professor
Lee Newman
Steering Committee Member
William Powell
Steering Committee Member
Mark Bremer
Abstract
Bacterial endophytes colonize total plant tissue without harming their hosts. One specific endophyte, Enterobacter sp. 638, is known to produce plant hormones which result in increased biomass (Taghavi et al., 2011). This study examined the effects of E. 638 inoculation and stress on growth of tomatoes (Solanum lycopersicum). Stressed inoculated plants produced significantly more fruit, and flowered, set fruit and ripened earlier than uninoculated plants. Stressed inoculated plants also had 30% larger root mass; however inoculation had no significant impact on unstressed plants. In addition, qPCR was used to measure the effects of inoculation on relative expression of certain genes in tomato root tissue. Inoculated plants had significantly higher transcript levels of ethylene signaling protein (EIN2), cytokinin receptor protein (CRE1) and cell wall relaxing protein (EXPA4) compared to uninoculated controls. These results may have major implications to agriculture by potentially enabling sustainable crop production under stressful conditions.
Recommended Citation
Fanfan, Gabrielle, "Growth and molecular responses of Solanum lycopersicum (Tomato) to Enterobacter sp. 638" (2017). Dissertations and Theses. 13.
https://digitalcommons.esf.edu/etds/13