Date of Award

Fall 12-4-2019

Document Type

Open Access Thesis

Degree Name

M.S. in Paper and Bioprocess Engineering

Department

Environmental and Forest Biology

Major Professor

Dr. Shijie Liu

Steering Committee Member

Dr. Bandaru V. Ramarao

Steering Committee Member

Dr. Chang Geun Yoo

Steering Committee Member

Dr. Deepak Kumar

Abstract

With the depleting petroleum reservation and the rapid growth of energy demand, people are eager for new renewable and environmentally friendly energy resource [1]. The biotransformation of biomass is one of the most efficient methods for an alternative resource [2]. In this study, Paulownia elongata hot-water wood extract was utilized as a carbon resource and induced Microbulbifer hydrolyticus to produce enzyme for cellulose and hemicellulose hydrolysis. The effect of temperature, starting time of feedstock (Paulownia elongata hot water wood extract) and feedstock flow rate were investigated. Based on the maximum cell concentration and protein concentration at stationary phase, bioreactor at 40°C had a higher productivity. For fed-batch, cell with Paulownia elongata hot-water wood extract had a higher productivity when the feedstock flow rate was 5 ml/h started 10 hours after inoculation. Enzymes produced by Microbulbifer hydrolyticus were also studied, cellulase and hemicellulase at pH 6, 40°C had a higher enzyme activity.

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