Date of Award

Spring 4-6-2018

Semester of Degree

May

Document Type

Open Access Dissertation

Degree Name

Ph.D. in Paper and Bioprocess Engineering

Department

Paper & Bioprocess Engineering

Major Professor

Shijie Liu

Steering Committee Member

Gary M. Scott

Steering Committee Member

Jianlin Xu

Steering Committee Member

Biljana Bujanovic

Steering Committee Member

Susumu Ikuta

Abstract

It has been well recognized that biologics are efficient for cancer and immune disease treatment. Kinetic modeling is to mathematically model or to quantitatively illustrate how reactions occur in a biological or chemical process. A systematic study and understanding of kinetic model and its application in the biopharmaceutical industry are important for both scientific research and industrial technology development. This work consists of six chapters. First, a review of kinetic modeling and its application for cell culture was introduced. Second, the current status of biologics development and screening strategies of biomarkers and indications were discussed. Third, one experimental and kinetic modeling study for the temperature effects and temperature shift strategy development was presented. Forth, novel kinetic models were built up and applied to elucidate lactate dehydrogenase catalyzed reactions, which is a crucial metabolic process within tumor cells and Chinese hamster ovary cells. In the fifth chapter, strategies of biologics quality control via process development were briefly summarized. And finally, summary and outlook were made based on the above five chapters. Though kinetic modeling is not a FDA request tool, kinetic data are required for regulation approval of new drug discovery and process development. These data are applicable for a rapidly screening of the best biologics and the optimized manufacturing process with little extra cost via kinetic modeling. This work is potentially beneficial for speeding up and better understanding of the current biologics development in biopharmaceutical industry.

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