Date of Award


Document Type



Environmental and Forest Biology

Thesis Advisor

William M. Shields


The ability of the flocculin (FLO) gene family, from the yeast Saccharomyces cerevisiae, to induce flocculation in green algae was investigated. Three genes of interest, FLO9, FLO10, and FLO11, were transformed into the high transgene-expressing Chlamydomonas reinhardtii strains, UVM4 and UVM11. Successfully transformed strains that contained the FLO9 gene were identified and confirmed by PCR. Of these, several showed a potential flocculating phenotype. Western Blot analysis on soluble extracts from the transformed strains identified candidate protein bands of the expected molecular weight in two of the transformed strains. Transformation and identification of transformants containing the FLO10 and FLO11 genes is in progress. Availability of naturally flocculating algal strains is expected to reduce the overall cost of biofuel production from algae.

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Botany Commons