Date of Award
Environmental and Forest Biology
William M. Shields
The ability of the flocculin (FLO) gene family, from the yeast Saccharomyces cerevisiae, to induce flocculation in green algae was investigated. Three genes of interest, FLO9, FLO10, and FLO11, were transformed into the high transgene-expressing Chlamydomonas reinhardtii strains, UVM4 and UVM11. Successfully transformed strains that contained the FLO9 gene were identified and confirmed by PCR. Of these, several showed a potential flocculating phenotype. Western Blot analysis on soluble extracts from the transformed strains identified candidate protein bands of the expected molecular weight in two of the transformed strains. Transformation and identification of transformants containing the FLO10 and FLO11 genes is in progress. Availability of naturally flocculating algal strains is expected to reduce the overall cost of biofuel production from algae.
Connolly, Morgan P., "Introduction of Yeast Genes into the Green Alga, Chlamydomonas reinhardtii, to Promote Algal Flocculation" (2014). Honors Theses. 41.